I-Brucerlla Antiy Elisa Kit

Ukucaciswa

 

IINKQUBO ZOKUVAVANYA NOLWAZI

 

Inyathelo loku-1 Inkqubo yovavanyo

1.1 Ukuhlakulela isisombululo seSisombululo

Wukugxininisa wUlwelo lwe-Alishing:Ngaphambi kokusetyenziswa, isisombululo sokuhlambela kufuneka sibuyiselwe kubushushu begumbi (20-25 degree) kwaye bangcungcutheke ngokulinganayo ukuze bangenise iyunifomu. Emva koko kutya izihlandlo ezingama-20 isisombululo sokuhlambela esinamanzi acocekileyo (umzekelo, isisombululo sokuhlamba i-5ml esigxininisiweyo songezwa kumanzi angama-95ml), kwaye ulwelo oluhlangeneyo lunokugcinwa kwi-2-8 idigri kangangeentsuku ezisi-7.

1.2 Ukulungiselela kwesampulu

I-①whole igazi lithathwe, i-Isterrifugd nge-4000rpm yemizuzu eli-10, kwaye i-Superntuant yaqokelelwa. I-serum inokuphakanyiswa ngokwendalo ngaphandle kwe-hemolysis.

Ubukhulu besampulu buninzi, ukwenzela ukuqinisekisa ukuba ixesha lovavanyo lokuphendula liyahambelana. Hlanganisa isampulu ye-serium ngesisombululo sokusebenza kwi-1: 1 kwi-diltelt ipleyiti ({4}} Isisombululo esisebenzayo).

1.3 Inkqubo yovavanyo

徽标 Kile i-Enzyme Labelsplatt (ngokwenani leesampulu, zinokubangelwa kwaye zisetyenziswe izihlandlo ezininzi), zisebenzise i-Wells ezimbini, zongeza i-100 yomxube we-intshi kwi-enzyme label mncinci. Ngobuthathaka shake isampulu equleni, usebenzisa ifilimu yeplate yokutywina ukugubungela ipleyiti, kwaye ifakwe kwinqanaba lama-37 kangangemizuzu engama-30.

②open ifilimu yokutywina, tyhila isisombululo kwiplate kakuhle, yongeza i-300μl yokuhlamba ulwelo kwiqula ngalinye, emva koko ulahle ulwelo equleni. Phinda amanyathelo angentla izihlandlo ezi-5, kwaye ekugqibeleni pat kwiphepha elicocekileyo.

③DI-ilzyme enzyme:Hlala i-enzyme quate (50 ×) 50 (umzekelo: 20ul enzyme + 980 + 980 i-UL ye-UL ye-UL i-UL i-UL ye-UL

I-④ADD ye-100 ye-enzyme ivumelana nayo nganye, gubungela ipleyiti, kwaye ifakwe kwinqanaba lama-37 kangangemizuzu engama-30.

Iplate ye-enzyme ye-enzyme, umsebenzi othile uyafana nale ②.

I-⑥ADD 100 i-100 i-100 i-⑥-100 i-100 i-quable i-quight nganye, igubungele iplate kwaye ifakwe imizuzu eli-10 kwinqanaba le-37 kude nokukhanya.

I-STADD ye-50μ yeka kwi-iquat nganye, kwaye ichonge isiphumo ngaphakathi kwemizuzu emi-5.

 

Inyathelo 2 mphumo

 

Ixabiso le-OD450 le-OD450 ye-HIV ifundwe kumfundi we-96.

I-2.1 ukuqinisekiswa

 

Ixabiso eliphakathi le-OD450NM lolawulo oluqinisekileyo yayingu-0.35 kunye nexabiso le-OD450NM le-OD450MM yolawulo olubi / ulawulo oluhle lwalungaphezulu okanye lilingana no-3.5 iziphumo zovavanyo bebesebenza. Ngaphandle koko, uvavanyo kufuneka luphindwe.

2.2 Indlela yokubala

Kwisampulu ngasinye, ukubala i-PI (i-Blockingr iTutyiwe) ngolu hlobo lulandelayo usebenzisa isampulu efanelekileyo kunye neenqobo zokulawula:

2.3 resultret inter

Xa i-PI <20%, igwetywa njengengalunganga.

Xa i-PI> 30%, igwetywa njengento elungileyo.

Xa i-20% engaphantsi okanye ilingane ne-PI ngaphantsi okanye ilingana nama-30%, igwetywa njengekrokrelweyo, kwaye iphakamisa ukuphinda iSampulse kwaye iphakamise iveki kamva.

Iifayile ezishisayo: I-Brucella Antitdy Elica Kit, China Brucella Antitl One Assovers Abathengisi, abavelisi, umzi-mveliso