Uvavanyo lweAntigen yeAvian Leukemia

Inkcazo

Uvavanyo lweAntigen yeAvian Leukemia

Ukusetyenziswa okucetywayo: ukukhangela umgangatho we-Avian Leukemia virus antigen (ALV Ag) kwiserum yentaka, iplasma, izicubu zesilonda okanye

iimfihlo ezivela kwi-cloaca.

Umgaqo: Uvavanyo lwe-immunochromatographic.

Umzekelo: i-serum yeentaka, iplasma, izicubu zesilonda okanye iimfihlo ezivela kwi-cloaca

Izixhobo ezinikezelweyo:

Iipowutshi ezili-10 zovavanyo, ezinamakhadi kunye needropha ezilahlwayo

Iibhotile ezili-10 zesithinteli sovavanyo

Iingxowa ezili-10 zeswab stick

1 iphakheji ifake

Imiboniso eyodwa:

1.Uvakalelo oluphezulu kunye nokuChaneka.

2.Inyathelo elinye{1}inkqubo yovavanyo

3.Iziphumo ezichanekileyo

INKQUBO YOVAVANYO

-Qokelela i-serum yentaka okanye iplasma kwityhubhu ye-centrifuge. Qinisekisa ukuba lo mzekelo ucocekile kwaye awufihli. Dlulisa amathontsi ama-3 (malunga ne-100μL) yomzekelo kwityhubhu yesithinteli sovavanyo kwaye uwaxube kakuhle. Sebenzisa umxube ekuvavanyeni.

{{0}Ukuba umfuziselo zizihlunu zesilonda, qokelela inxalenye yethishu kwisithinteli sovavanyo. Dlulisa kwaye utyumze izicubu kwaye wenze izicubu zixutywe kangangoko kunokwenzeka kwisithinteli sovavanyo.

Sebenzisa umxube we-supernatant ekuvavanyeni.

-Ukuba lo mzekelo uphuma kwi-cloaca, qokelela incindi yentaka okanye ilindle ngeswab kwaye ufake iswab kumbhobho onikiweyo wesithinteli. Yivuselele ukufumana isampulu esebenzayo yotsalo.

-Khupha isixhobo sovavanyo kwisingxobo sefoil kwaye usibeke ngokuthe tye.

-Funxa isampulu enyangwayo yokutsalwa kwityhubhu yesithinteli sovavanyo kwaye ubeke amathontsi ama-3 kwisampulu kakuhle "S" yesixhobo sovavanyo.

-Tolika iziphumo kwimizuzu emi-5-10. Isiphumo emva kwemizuzu eli-10 sithathwa njengesingekho mthethweni.

UKUTOLIKWA KWEZIPHUMO

Okuhle (+): Ubukho bazo zombini umgca "C" kunye nomgca wezowuni "T", nokuba umgca T ucacile okanye awucacanga.

Engalunganga ({0}}): Kuvela umgca C ocacileyo kuphela. Akukho mgca T.

Ayisebenziseki: Akukho mgca mbala uvelayo kwindawo C. Kungakhathaliseki ukuba umgca we-T uyavela.

Ulwazi lweodolo

Iifayile ezishisayo: I-avian leukemia virus ye-antigen test, i-China avian leukemia virus i-antigen test ababoneleli, abavelisi, umzi-mveliso