UVavanyo lwe-Ovine Lokukhulelwa Kwangexesha-

I-Ovine yokukhulelwa kwangethuba-Uvavanyo luvavanyo lwe-lateral flow immunochromatographic ukuze kufunyaniswe ubungakanani boxinzelelo lwe-ovine progesterone kwi-ovine serum okanye i-plasma specimen. Ngokulinganisa ukuxinwa kweprogesterone kwi-specimen, le mveliso yenzelwe ukuba ibe nomda wokufumanisa i-3ng / ml kwaye ifanelekile ukuvavanya okuqhubekayo kwiintsuku ezingama-20-25 emva kokutshatyalaliswa. I-Ewe enamanqanaba eprogesterone Enkulu okanye elingana ne-3ng/ml phakathi kweentsuku ezingama-20 ukuya kwezingama-25 emva kokuba idliwe ifunyaniswe ukuba ikhulelwe.<3ng/mL at any time during thisperiod,theewe was diagnosed as non-pregnant.The Ovine early pregnancy Quanti-Test can be applied to FIC-Q10oN Dry Immunoassay Analyzer for result reading.

Inkcazo

Ukusetyenziswa okucetywayo: ukubonwa kwe-ovine progesterone concentration

Umgaqo: Uvavanyo lwe-immunochromatographic.

Umzekelo: i-ovine serum okanye iplasma

Izixhobo ezinikezelweyo:

10 Ovine ukukhulelwa kwangethuba isixhobo sovavanyo lwe-Quanti
10 Centrifuge ityhubhu
20 incam yepipette elahlayo
2 ityhubhu yesithinteli sovavanyo (1500μL)
1 Umyalelo wokusetyenziswa

Imiboniso eyodwa:

Uvakalelo oluphezulu kunye nokuChaneka.

Uvakalelo olunxulumeneyo: 97.50%

Iinkcukacha Ngokuhambelana: 97.06%

Ukuchaneka okuhambelanayo: 97.25%

Inkqubo yoVavanyo

1. Vumela zonke iimathiriyeli, ukuquka umfuziselo kunye nesixhobo sovavanyo, ziphinde zibe 15 - 25 isidanga phambi kokusetyenziswa.

2. Vula iFIC - Q100N Dry Immunoassay Analyzer kwaye ujonge isimo.

3. Sebenzisa i-scanner esiza kunye ne-analyzer ukuskena ikhowudi ye-QR ngaphakathi kwebhokisi ukuze usebenzise i-curve yokulinganisa kwi-analyzer.

4. Khupha ikhadi lovavanyo kwisingxobo se-foil kwaye ulifake kwi-slot yekhadi lovavanyo lwe-analyzer. Gcina isampula kakuhle yekhadi lovavanyo lijonge phezulu kwaye ushiye isampulu kakuhle ngaphandle.

5. Lungisa isethingi yevolumu ye-pipette kwi-100μL. Zoba i-100μL yesithinteli sovavanyo kwaye wongeze kwityhubhu ye-centrifuge.

6. Faka enye incam yepipette, kwaye ulungelelanise ukuseta umthamo we-pipette kwi-50μL. Zoba i-50μL yomzekelo kwaye wongeze kwityhubhu ye-centrifuge kwaye udibanise isampuli kakuhle.

7. Lungisa isethingi yevolumu ye-pipette kwi-100μL. Zoba i-100μL yesampulu exutyiweyo kwisampulu yequla lekhadi lovavanyo.

8. Cofa [UVAVANYO OLUMGANGATHO] kwisikrini. Isiphumo siya kuboniswa kwimizuzu eyi-15.

Ukutolikwa kweziPhumo

Funda ixabiso loxinaniso lweprogesterone ye-ovine eboniswe kwi-analyzer (3ng / mL ~ 50ng / mL). Uvavanyo oluqhubekayo lwenziwa ngosuku lweentsuku ezingama-20 ukuya kwezingama-25 emva kokufakwa kwamadlozi. Kwimeko nganye, iimazi ezinamaqondo eprogesterone Enkulu okanye elingana ne-3ng/mL zafunyaniswa zikhulelwe. Ukuba inqanaba leprogesterone lalinjalo<3ng/mL at any time during this period, the patient was diagnosed as non-pregnant.
Okungasebenziyo: Akukho mgca mbala uvelayo kumgca C, nokuba umgca uvela kumgca T. Ibonisa ukuba ukusebenza okungalunganga kunokwenzeka kwaye iziphumo zovavanyo azivumelekanga. Phinda uvavanyo ngovavanyo olutsha olukhawulezayo.

Ulwazi lweodolo

Iifayile ezishisayo: Uvavanyo lokukhulelwa kwe-ovine kwangoko -, i-China ovine yokukhulelwa kwangoko quanti{1}} uvavanyo lwababoneleli, abavelisi, umzi-mveliso